Enabling Precise Single-Cell Segmentation
The Cell Boundaries Assay Kit delivers fluorescently labeled antibodies for cell boundary staining optimized by our robust validation workflow, enabling clear delineation of individual single cells within intact tissue architecture.
By improving segmentation accuracy, the kit ensures each protein signal is correctly assigned to its cell of origin, resulting in cleaner, more reliable data for cell type classification, spatial mapping, and neighborhood analysis. The panel can be expanded by pairing with additional VistaPlex Multiplexing Assay Kits or your markers of choice.
The VistaPlex Cell Boundaries Assay Kit enables automated staining and imaging of 10 human FFPE samples for 4 cell segmentation biomarkers using the CellScape Precise Spatial Proteomics platform. An online protocol is available to guide consistent and reliable execution of the multiplexed assay.
How it Works
The VistaPlex Cell Boundaries Assay Kit enables multiplexed detection and definition of cell boundaries directly in situ to deliver accurate protein assignment and cell type classification across FFPE tissue. Each panel is fully validated for use with EpicIF™ chemistry and the CellScape™ platform, ensuring consistent staining, robust signal removal, and high-quality single-cell proteomic data
Read the VistaPlex™ Cell Boundaries Assay Kit validation report to learn more.
Validation is performed on human FFPE tonsil as a positive control, as well a multiple fundamental disease states.
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