VistaPlex™ Spatial Immune Profiling Assay Kit

For the CellScape™ platform.
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Fundamental Spatial Immunophenotyping of the Immune Microenvironment

Providing a building blog for essential spatial discovery of the immune microenvironment, the Spatial Immune Profiling Assay Kit includes a panel of antibodies curated and optimized by our robust validation workflow. The panel targets key immune biomarkers and can be expanded by pairing with additional VistaPlex Multiplexing Assay Kits.

The VistaPlex Spatial Immune Profiling Assay Kit enables automated staining and imaging of 10 human FFPE samples for 17 biomarkers using the CellScape Precise Spatial Proteomics platform. An online tool is available to guide consistent and reliable execution of the multiplexed assay.

How it Works

The VistaPlex Spatial Immune Profiling Assay Kit enables multiplexed detection of key immune markers directly in situ, delivering quantitative, spatially resolved phenotypes across FFPE tissue. Each panel is fully validated for use with EpicIF™ chemistry and the CellScape™ platform, ensuring consistent staining, robust signal removal, and high-quality single-cell proteomic data.

Read the VistaPlex™ Spatial Immune Profiling Kit validation report to learn more.

01:

Validated Antibodies

The kit includes 17 validated, fluorescently labeled antibodies to support tissue immunophenotyping.

02:

Optimized Protocol

An online protocol is available to guide consistent and reliable execution of the multiplexed assay.

03:

Reliable Performance

Antibodies for the multiplex panel were validated using eight human tonsil FFPE tissue samples to ensure specificity, sensitivity, and reproducibility. Additional FFPE tissues were validated for broad sample suitability.

04:

Expandable Assays

Expandable Assays. Expand the Spatial Immune Profiling Assay Kit with modular VistaPlex Assay Kits to build a customized Precise Spatial Proteomics panel. Compatible kits can be run on the same sample, and the CellScape workflow lets you seal, store, and revisit the same slide. Add new biomarkers later to drive data-driven panel expansion and make the most of precious samples.

Reliably Assess the Immune Microenvironment
Intra- and Inter-Site Validation
Qualitative Suitability and Specificity Assessment
Quantitative Reproducibility Assessment
Tissue Type Suitability
Reliably Assess the Immune Microenvironment
Full overview of a tonsil sample used in validation testing. The red boxes indicate the germinal center (1) and squamous epithelia (2) regions shown in enlarged images.

Enlarged images show individual antibody stains in grayscale and multicolor counterstain examples.
Intra- and Inter-Site Validation

Following clone selection and assay design, the multiplex panels are validated for performance by specificity, sensitivity, reproducibility, and suitability measurements. Validation experiments are completed using serial tissue sections from multiple relevant tissues, on different CellScape instruments, in different laboratory sites, and with staining scored by multiple scientists
Qualitative Suitability and Specificity Assessment
Average scores from technical replicates of human FFPE Tonsil are visualized in a heatmap (A, green=pass, red=fail) and a radar plot (B). n = 8 samples scored by four independent judges.
Quantitative Reproducibility Assessment
Cell density measurements for each stain across three technical replicates of human FFPE tonsil
Tissue Type Suitability

Validation is performed on human FFPE tonsil as a positive control, as well a multiple fundamental disease states.

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Related Resources

Product Bulletin VistaPlex™ Validation Report – Spatial Immune Profiling Kit
App Note/Tech Note VistaPlex™ Assay Kit Design and Validation Process
Event Building High-Plex Spatial Proteomic Panels – A Simplified, Flexible Workflow Using the CellScape™ Platform
Poster Expandable Immunofluorescence Assays with Next-Generation VistaPlex Immune Profiling Kits

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